%0 Journal Article
%T Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum<br>日本血吸虫真核翻译起始因子5基因的cDNA克隆和免疫保护功能分析(英文)
%A CHENG Guo-feng
%A LIN Jiao-jiao
%A SUN Jun
%A LI Hao
%A ZHU Chuan-gang
%A ZHOU Yuan-cong
%A CAI You-min
%A <br>程国锋
%A 林矫矫
%A 孙军
%A 李浩
%A 朱传刚
%A 周元聪
%A 蔡幼民
%J 动物学研究
%D 2005
%I Chinese Academy of Sciences
%X The expressed sequence tag of eukaryotic translation initiation factor 5 (elF5) from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioin-formatics method. The overlapping sequences were assembled into one that includes the complete open reading frame (GenBank accession number: AY686501). The full-length cDNA of SjelF5 was cloned into a pET-28c( ) vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coli. The recombi-nant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.
%K Schistosoma japonicum
%K eIF5
%K Expression
%K Vaccine<br>日本血吸虫
%K 真核翻译起始因子5(eIF5)
%K 表达
%K 疫苗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=4C529C4E3E0BF33A&yid=2DD7160C83D0ACED&vid=96C778EE049EE47D&iid=94C357A881DFC066&sid=F9A6B6F259CE5121&eid=5A751AE9FA58A3FB&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=12