STUDIES ON THE THROMBIN-LIKE PRINCIPLES OF Agkistrodon acutus VENOM III. STUDIES ON HEMOSTATIC EFFECT OF HEMOCOAGULASE
尖吻蝮(Agkistrodon acutus)蛇毒凝血酶样成分的研究——Ⅲ.蛇毒凝血酶止血效应的研究

Thrombin-like enzymes (TLE3, TLE4) had been isolated and purified from the venom of Agkistrodon acutus. With 2- 3 NIH units/kg in vivo the result showed that the blood coagulation time of rabbits was shortened in 1/3-1/2. In vivo eyperiments (2NIH or 3NIH/kg) after 1 hr TLE began to show effect, after 2 - 4 hr the effect was most strong, after 12 hr there was no effect. The properties of TLE is similar to hemocoagulase from venom Bathrope jararaca. The experiments showed that TLE 3 and 4 of A. acutus can used as hemostatic agents.