Study on Isolation, Purification and Antitumor Activity of Polysaccharides from Porphyra yezoensis
条斑紫菜多糖的分离纯化与抗肿瘤活性的初探

The polysaccharide from Porphyra yezoensis(PYP) was purified and analyzed by biochemical techniques. The effect of PEP on the proliferation of MCF-7 was investigated in vitro. Methods: The purified polysaccharide from Porphyra yezoensis was obtained by DEAE-cellulose and Sephadex G-200 column chromatography. The effect of PEP on the proliferation of MCF-7 was investigated in vitro by MTT method. Two components, PY-D1 and PY-D2, were isolated from the Porphyra yezoensis polysaccharide by ion-exchange chromatography DEAE-52. Two polysaccharides, PY-G1 and PY-G2, were further isolated from PY-D2 Ueda by gel filtration Sephadex G-200. PY-G was identified by UV and IR spectra. All of components could inhibit the proliferation of MCF-7. Results: It was found polysaccharides of Porphyra yezoensis had antitumor activity for MCF-7 cells.