%0 Journal Article
%T Study on Isolation, Purification and Antitumor Activity of Polysaccharides from Porphyra yezoensis<br>条斑紫菜多糖的分离纯化与抗肿瘤活性的初探
%A GU Jia-wen
%A ZHANG Lu-xi
%A XU Hong-li
%A GUO Ting-ting
%A HE Pei-min
%A <br>顾佳雯
%A 张陆曦
%A 徐红丽
%A 郭婷婷
%A 何培民
%J 中国生物工程杂志
%D 2007
%I 
%X The polysaccharide from Porphyra yezoensis(PYP) was purified and analyzed by biochemical techniques. The effect of PEP on the proliferation of MCF-7 was investigated in vitro. Methods: The purified polysaccharide from Porphyra yezoensis was obtained by DEAE-cellulose and Sephadex G-200 column chromatography. The effect of PEP on the proliferation of MCF-7 was investigated in vitro by MTT method. Two components, PY-D1 and PY-D2, were isolated from the Porphyra yezoensis polysaccharide by ion-exchange chromatography DEAE-52. Two polysaccharides, PY-G1 and PY-G2, were further isolated from PY-D2 Ueda by gel filtration Sephadex G-200. PY-G was identified by UV and IR spectra. All of components could inhibit the proliferation of MCF-7. Results: It was found polysaccharides of Porphyra yezoensis had antitumor activity for MCF-7 cells.
%K MCF-7<br>条斑紫菜多糖
%K 分离纯化
%K MTT法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=068DCD7403CAD48C&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=DF92D298D3FF1E6E&sid=771152D1ADC1C0EB&eid=318E4CC20AED4940&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=17