The Research on Expression and Purification of a Chimeric anti-p185 antibody
抗P185工程抗体表达与纯化的研究

TThe Her-2 proto-oncogene encodes a 185kD transmenbrane glycoprotein p185 which has intrinsic tyrosine kinase activity. It is overexpressed in several malignant human tumors like breast cancer. We have constructed a chimeric antibody by assembling a single-chain Fv antibody and a human IgG1 Fc fragment. This chimeric antibody reacts with tumor surface antigen p185c-erbB-2 specifically. In order to put the antibody into clinical application, we use two steps purification method to attain the antibody's purity more than 95%. Then we found both the lyophilized pharmaceutical formulations of the antibody. The formulations can keep the stability and activity of the antibody for at least 1 year. These results were the foundation of the chimeric antibody for cancer therapy.