%0 Journal Article
%T The Research on Expression and Purification of a Chimeric anti-p185 antibody<br>抗P185工程抗体表达与纯化的研究
%A QIAO Jing-juan
%A LIU Hai-bo
%A ZHU Juan-juan
%A CHENG Lian-sheng
%A LIU Jing
%A <br>乔婧娟
%A 刘海波
%A 朱娟娟
%A 程联胜
%A 刘兢
%J 中国生物工程杂志
%D 2006
%I 
%X TThe Her-2 proto-oncogene encodes a 185kD transmenbrane glycoprotein p185 which has intrinsic tyrosine kinase activity. It is overexpressed in several malignant human tumors like breast cancer. We have constructed a chimeric antibody by assembling a single-chain Fv antibody and a human IgG1 Fc fragment. This chimeric antibody reacts with tumor surface antigen p185c-erbB-2 specifically. In order to put the antibody into clinical application, we use two steps purification method to attain the antibody's purity more than 95%. Then we found both the lyophilized pharmaceutical formulations of the antibody. The formulations can keep the stability and activity of the antibody for at least 1 year. These results were the foundation of the chimeric antibody for cancer therapy.
%K Chimeric antibody Affinity purification Cation exchange purification Stability<br>嵌合抗体
%K 亲和纯化
%K 离子交换纯化
%K 稳定性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=7A29F6A66B67AC59&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=9CF7A0430CBB2DFD&sid=9971A5E270697F23&eid=42425781F0B1C26E&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=2&reference_num=16