Analysis of antigen motifs presented by HLA- B27 using combinatorial peptide libraries
用组合肽库技术分析HLA-B27的抗原提呈模式

Analysis of antigen motifs presented by HLA molecules is important for the study of autoimmune disease and cancer vaccine.Using the method of combinatorial peptide libraries,nineteen ORX7 peptide sublibraries were synthesized.Through a competition binding assay between fluorescein labeled reference peptide and peptide sublibraries,the antigen motifs presented by HLA-B27 were investigated.The results revealed that the binding proportions of FL-peptide after competed with different peptide sublibraries were similar,suggest that P1 was non-anchor residue for HLA-B27 peptide binding.HLA-B*2704 and-B*2705 found in Chinese population existed some differences about their binding motifs on P1 position.D and E are easier to binding with HLA-B*2704 compared to B*2705,K is easier to binding with HLA-B*2705 compared to B*2704.This investigation provided some clues for exploring the mechanism of association between HLA-B27 and ankylosing spondylitis.