%0 Journal Article
%T Analysis of antigen motifs presented by HLA- B27 using combinatorial peptide libraries<br>用组合肽库技术分析HLA-B27的抗原提呈模式
%A JIANG Li-hua
%A XU Hong-yan
%A ZHANG Hai-jin
%A SHI Gui-ying
%A SHI Xue-geng
%A LI Wei-yi
%A <br>蒋黎华
%A 徐红岩
%A 张海静
%A 史桂英
%A 石学耕
%A 李伟毅
%J 中国生物工程杂志
%D 2007
%I 
%X Analysis of antigen motifs presented by HLA molecules is important for the study of autoimmune disease and cancer vaccine.Using the method of combinatorial peptide libraries,nineteen ORX7 peptide sublibraries were synthesized.Through a competition binding assay between fluorescein labeled reference peptide and peptide sublibraries,the antigen motifs presented by HLA-B27 were investigated.The results revealed that the binding proportions of FL-peptide after competed with different peptide sublibraries were similar,suggest that P1 was non-anchor residue for HLA-B27 peptide binding.HLA-B*2704 and-B*2705 found in Chinese population existed some differences about their binding motifs on P1 position.D and E are easier to binding with HLA-B*2704 compared to B*2705,K is easier to binding with HLA-B*2705 compared to B*2704.This investigation provided some clues for exploring the mechanism of association between HLA-B27 and ankylosing spondylitis.
%K HLA-B27<br>组合肽库
%K 抗原提呈
%K 竞争结合试验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=2E45FADC6A7D17F7&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=0B39A22176CE99FB&sid=BE33CC7147FEFCA4&eid=D997634CFE9B6321&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=11