Construction and expression analyzing of bicistronic vector based IERS from FMDV
基于FMDV IRES的双顺反子载体的构建及体外表达分析

The internal ribosome entry site (IRES) element from FMDV was amplified by RT-PCR,and was cloned into pcDNA3.1( ) vector,resulted in a bicistronic vector.To determine whether the bicistronic mRNAs from the vector can be translated,the enhanced green fluorescent protein (EGFP) gene was cloned into the downstream of the IERS element,then BHK-21 cells was transfected by the recombinant plasmid.The green fluorescence in the transfected cells was observed under a fluorescence microscope equipped with a video documentation system.And the expressional efficiency was analyzed with flow cytometry (FCM).The results show that the IRES element from FMDV has the role of initiating CAP-independent translation,and lay foundation for researching function of the element and interrelated proteins.It would be potential for expressing target gene by the bicistronic vector.