%0 Journal Article
%T Construction and expression analyzing of bicistronic vector based IERS from FMDV<br>基于FMDV IRES的双顺反子载体的构建及体外表达分析
%A ZHENG Hai-xue
%A GUO Hui-chen
%A JIN Ye
%A LIU Xiang-tao
%A XIE Qing-ge
%A <br>郑海学
%A 郭慧琛
%A 靳野
%A 刘湘涛
%A 谢庆阁
%J 中国生物工程杂志
%D 2007
%I 
%X The internal ribosome entry site (IRES) element from FMDV was amplified by RT-PCR,and was cloned into pcDNA3.1( ) vector,resulted in a bicistronic vector.To determine whether the bicistronic mRNAs from the vector can be translated,the enhanced green fluorescent protein (EGFP) gene was cloned into the downstream of the IERS element,then BHK-21 cells was transfected by the recombinant plasmid.The green fluorescence in the transfected cells was observed under a fluorescence microscope equipped with a video documentation system.And the expressional efficiency was analyzed with flow cytometry (FCM).The results show that the IRES element from FMDV has the role of initiating CAP-independent translation,and lay foundation for researching function of the element and interrelated proteins.It would be potential for expressing target gene by the bicistronic vector.
%K IRES<br>口蹄疫病毒
%K 双顺反子
%K 双顺反子载体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=EC4D192E447D6AB4&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=5D311CA918CA9A03&sid=771469D9D58C34FF&eid=27746BCEEE58E9DC&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=20