Study on the Real-time PCR Quantity Detecting Methods of Genetic Modified Tobacco
转基因烟草荧光定量检测方法研究

The TaqMan primers and probes for real-time PCR were designed according to the sequence of 35S promoter, NOS terminator,GUS reporter and NPTII selection gene. The most suitable TaqMan primer and probe, PCR system and PCR condition were suggested from the experiments on GMT real-time PCR quantity detecting methods using DNA Engine Opticon 2 real-time PCR. The Z-score from CORESTA genetic modified tobacco testing ring trial confirmed the accuracy and stability of this methods.