%0 Journal Article
%T Study on the Real-time PCR Quantity Detecting Methods of Genetic Modified Tobacco<br>转基因烟草荧光定量检测方法研究
%A GUO Zhao-kui YAN Pei-qiang WAN Xiu-qing LI Li-jie YANG Qian
%A <br>郭兆奎
%A 颜培强
%A 万秀清
%A 李丽杰
%A 杨谦
%J 中国生物工程杂志
%D 2005
%I 
%X The TaqMan primers and probes for real-time PCR were designed according to the sequence of 35S promoter, NOS terminator,GUS reporter and NPTII selection gene. The most suitable TaqMan primer and probe, PCR system and PCR condition were suggested from the experiments on GMT real-time PCR quantity detecting methods using DNA Engine Opticon 2 real-time PCR. The Z-score from CORESTA genetic modified tobacco testing ring trial confirmed the accuracy and stability of this methods.
%K Tobacco GMO testing Real-time PCR<br>烟草
%K 转基因检测
%K 荧光定量PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=F2C6BF8F72ED4F96&yid=2DD7160C83D0ACED&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=228A710F49B6CE58&eid=E203FB1A272C9DD2&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=8&reference_num=8