Biotransformation of Eight Active Constituents by hairy Roots of Polygonum multiflorum Thunb
转基因何首乌毛状根对8种活性成分的生物转化研究

Objective: To investigate the biotransformation of eight active constituents chemicals by hairy roots of Polygonum multiflorum Thunb. Methods: Active compounds were added to the culture system of hairy roots of P. multiflorum after precultured for 9 d, then they were co-cultured for another 7 d. The products were isolated and purified by column chromatography. Results: Three of the eight active constituents were identified to be biotransformed, which were furannoligularenone (6), 1,4-benzenediol (7) and artemisinin (8). HPLC analysis of the biotransformation of 6 showed two new peaks except the peak of substrate, then the corresponding two compounds were obtained; Substrate 7 was converted into arbutin. In the case of 8, there were two new peaks and the corresponding two monomers have been also isolated. Conclusion: The result showed that there were several enzymes in the culture system of P. multiflorum hairy roots, which could processed glycosylation, oxidation, reduction and hydroxylation reactions.