%0 Journal Article
%T Biotransformation of Eight Active Constituents by hairy Roots of Polygonum multiflorum Thunb<br>转基因何首乌毛状根对8种活性成分的生物转化研究
%A YAN Chun-yan
%A MA Wei-li
%A LIANG Jian
%A LU Hua-chong
%A ZHANG De-zhi
%A YU Rong-min
%A <br>严春艳
%A 马伟丽
%A 梁建
%A 吕华冲
%A 张德志
%A 于荣敏
%J 中国生物工程杂志
%D 2008
%I 
%X Objective: To investigate the biotransformation of eight active constituents chemicals by hairy roots of Polygonum multiflorum Thunb. Methods: Active compounds were added to the culture system of hairy roots of P. multiflorum after precultured for 9 d, then they were co-cultured for another 7 d. The products were isolated and purified by column chromatography. Results: Three of the eight active constituents were identified to be biotransformed, which were furannoligularenone (6), 1,4-benzenediol (7) and artemisinin (8). HPLC analysis of the biotransformation of 6 showed two new peaks except the peak of substrate, then the corresponding two compounds were obtained; Substrate 7 was converted into arbutin. In the case of 8, there were two new peaks and the corresponding two monomers have been also isolated. Conclusion: The result showed that there were several enzymes in the culture system of P. multiflorum hairy roots, which could processed glycosylation, oxidation, reduction and hydroxylation reactions.
%K Transgenic Polygonum multiflorum
%K hairy root
%K biotransformation<br>转基因何首乌
%K 毛状根
%K 生物转化
%K 转基因
%K 何首乌
%K 毛状根
%K 活性成分
%K 生物转化
%K 研究
%K Polygonum
%K multiflorum
%K Hairy
%K Roots
%K Constituents
%K Active
%K 修饰
%K 羟基化
%K 氧化
%K 糖基化
%K 酶系统
%K 存在
%K 悬浮体系
%K 对苯二酚
%K 柱色谱法分离
%K 发生
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=C0AF5DAF3855EC3A8D2DC96557BC481D&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=E158A972A605785F&sid=46CB27789995047D&eid=35FC3610259C2B32&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=10