Construction of the expression vector of virus-like particles containing FMDV IRES RNA
含口蹄疫病毒IRES RNA病毒样颗粒表达载体的构建

The Coat protein and Maturase gene of E. coli bacteriophage MS2 was amplified by PCR, then the gene was cloned into pET32a to construct the intermediate vector pET32a-CP. The conservative sequence of FMDV internal ribosome entry site (IRES) was cloned into the downstream of pET32a-CP bacteriophage gene to construct the prokaryotic expression vector pCPES. The recombinant plasmid pCPES transformed into E. coli strain BL21 (DE3) was induced to express with 1mmol/L IPTG. The expression products were purified by sucrose density gradient centrifugation. The expression products observed by TEM were circular virus-like particles, and the diameter of these particles was about 26 nm.. The stability of virus-like particles was detected, and the virus-like particles was identified by RT-PCR. The results showed that the virus-like particles contain the FMDV IRES RNA and have good stability. The study shows that the virus-like particles have great prospect as the standard and quality control in the area of RNA virus detection.