%0 Journal Article
%T Construction of the expression vector of virus-like particles containing FMDV IRES RNA<br>含口蹄疫病毒IRES RNA病毒样颗粒表达载体的构建
%A DOU Min
%A ZHANG Guo-guang
%A YU Guang-fu
%A ZHANG Hong-xin
%A SHEN Ming-shan
%A CHEN Liang
%A <br>窦敏
%A 张国广
%A 于广福
%A 张红心
%A 沈明山
%A 陈亮
%J 中国生物工程杂志
%D 2007
%I 
%X The Coat protein and Maturase gene of E. coli bacteriophage MS2 was amplified by PCR, then the gene was cloned into pET32a to construct the intermediate vector pET32a-CP. The conservative sequence of FMDV internal ribosome entry site (IRES) was cloned into the downstream of pET32a-CP bacteriophage gene to construct the prokaryotic expression vector pCPES. The recombinant plasmid pCPES transformed into E. coli strain BL21 (DE3) was induced to express with 1mmol/L IPTG. The expression products were purified by sucrose density gradient centrifugation. The expression products observed by TEM were circular virus-like particles, and the diameter of these particles was about 26 nm.. The stability of virus-like particles was detected, and the virus-like particles was identified by RT-PCR. The results showed that the virus-like particles contain the FMDV IRES RNA and have good stability. The study shows that the virus-like particles have great prospect as the standard and quality control in the area of RNA virus detection.
%K MS2 bacteriophage Coat protein RNA virus Virus-like particles<br>MS2噬菌体
%K 外壳蛋白
%K RNA病毒
%K 病毒样颗粒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=93DFFB78CE5BF177&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=9CF7A0430CBB2DFD&sid=4AD960B5AD2D111A&eid=6209D9E8050195F5&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=12