Technologies for Surface Display of Lipase and its Application
脂肪酶的表面展示及其应用

Lipase is a kind of widely used hydrolase. Surface display is an efficient method of high-throughput screening for protein engineering of lipase. Besides, lipase displaying on surface of microorganism has many advantages, such as higher stability against high temperature and organic solvent, compared with free lipase, so the host strain displaying lipase can be used as whole-cell biocatalyst, which has some advantages compared with traditional immobilization of lipase. There are three kinds of host strain for displaying lipase: phage, bacteria and yeast. This article systematically describes surface display of lipase in the three display systems, and discusses their current uses and their possible trends in the future.