%0 Journal Article
%T Technologies for Surface Display of Lipase and its Application<br>脂肪酶的表面展示及其应用
%A LIU Wen-shan
%A YAN Yun-jun
%A <br>刘文山
%A 闫云君
%J 中国生物工程杂志
%D 2007
%I 
%X Lipase is a kind of widely used hydrolase. Surface display is an efficient method of high-throughput screening for protein engineering of lipase. Besides, lipase displaying on surface of microorganism has many advantages, such as higher stability against high temperature and organic solvent, compared with free lipase, so the host strain displaying lipase can be used as whole-cell biocatalyst, which has some advantages compared with traditional immobilization of lipase. There are three kinds of host strain for displaying lipase: phage, bacteria and yeast. This article systematically describes surface display of lipase in the three display systems, and discusses their current uses and their possible trends in the future.
%K Lipase Surface display High throughput screen whole-cell biocatalyst<br>脂肪酶
%K 表面展示技术
%K 高通量筛选
%K 全细胞催化剂
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=7A2E60F011FAB666&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=9CF7A0430CBB2DFD&sid=C3BF5C58156BEDF0&eid=331211A5F5616413&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=25