Isolation of proteins related to the dwarf mutant by two-dimensional gel electrophoresis in soybean
利用双向电泳技术分离大豆矮秆突变体相关蛋白

Dwarf is one of the major target traits in soybean breeding.Two dimensional electrophoresis (2-DE) was used to analyze the different proteins between the dwarf mutant (Dongze11) and its wild type (Dongnong42), which can be used to identified the gene related to the dwarf mutant. By combination and optimization of various conditions, a sample preparation method of 2-DE technique was established,which were used for further analyses. The total leaf proteins between mutant and wild soybean were compared with two-dimensional gel electrophoresis. There were 9 spots were notable detected, including 6 up-regulated proteins and 3 down-regulated proteins. The two dimensional electrophoresis (2-DE) system to analyze leaves was established. The similarity and difference of this system from the previous were discussed.