%0 Journal Article
%T Isolation of proteins related to the dwarf mutant by two-dimensional gel electrophoresis in soybean<br>利用双向电泳技术分离大豆矮秆突变体相关蛋白
%A Li-Xia CHEN Ying-hui LI Zhao-yang REN Rong-xia GUANG Zhang-qiong LIU Fu-cong ZHENG Zai-bin HAO Ru-zhen CHANG Li-juan QIU
%A <br>陈丽霞
%A 李英慧
%A 任朝阳
%A 关荣霞
%A 刘章雄
%A 郑服丛
%A 郝再彬
%A 常汝镇
%A 邱丽娟
%J 中国生物工程杂志
%D 2007
%I 
%X Dwarf is one of the major target traits in soybean breeding.Two dimensional electrophoresis (2-DE) was used to analyze the different proteins between the dwarf mutant (Dongze11) and its wild type (Dongnong42), which can be used to identified the gene related to the dwarf mutant. By combination and optimization of various conditions, a sample preparation method of 2-DE technique was established,which were used for further analyses. The total leaf proteins between mutant and wild soybean were compared with two-dimensional gel electrophoresis. There were 9 spots were notable detected, including 6 up-regulated proteins and 3 down-regulated proteins. The two dimensional electrophoresis (2-DE) system to analyze leaves was established. The similarity and difference of this system from the previous were discussed.
%K Soybean Dwarf Two dimensional electrophoresis (2-DE) Proteomics<br>大豆
%K 矮秆
%K 双向电泳
%K 蛋白质组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=4362C8AB07AF26B9&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=38B194292C032A66&sid=228A710F49B6CE58&eid=0D0D661F0B316AD5&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=19