The Effect of bFGF on the Proliferation and Diffrentiation of Growth-plate Chondrocytes
碱性成纤维细胞生长因子对生长板软骨细胞增殖与分化的影响

Objectives: To study the effect of basic fibroblast growth factor (bFGF) on the proliferation and differentiation in cultured growth-plate chondrocytes. Methods: Growth-plate chondrocytes were isolated from rabbit costal cartilage and cultured under low-serum condition in monolayers. Cell growth was detected by the improved MTT; the production of collagen was estimated according to the hydroxyproline content; ALP activity was measured by modified enzyme dynamical method. Result: The cultured chondrocytes in medium with bFGF at concentrations of 5 -100ng/ml were significantly more than those in control group, which reached the peak at the concentration of 25ng/ml. The synthesis of collagen was inhibited by bFGF at the concentration of above 25ng/ml. The activity of ALP was suppresseded by bFGF at the concentration of above 1ng/ml. Conclusion: bFGF stimulated the proliferation of growth-plate chondrocytes obviously, but inhibited the differentiation of growth-plate chondrocytes at high concentrations.