%0 Journal Article
%T The Effect of bFGF on the Proliferation and Diffrentiation of Growth-plate Chondrocytes<br>碱性成纤维细胞生长因子对生长板软骨细胞增殖与分化的影响
%A YU Li WU Feng-lin WANG Zhuo-yun
%A <br>宇丽
%J 中国生物工程杂志
%D 2005
%I 
%X Objectives: To study the effect of basic fibroblast growth factor (bFGF) on the proliferation and differentiation in cultured growth-plate chondrocytes. Methods: Growth-plate chondrocytes were isolated from rabbit costal cartilage and cultured under low-serum condition in monolayers. Cell growth was detected by the improved MTT; the production of collagen was estimated according to the hydroxyproline content; ALP activity was measured by modified enzyme dynamical method. Result: The cultured chondrocytes in medium with bFGF at concentrations of 5 -100ng/ml were significantly more than those in control group, which reached the peak at the concentration of 25ng/ml. The synthesis of collagen was inhibited by bFGF at the concentration of above 25ng/ml. The activity of ALP was suppresseded by bFGF at the concentration of above 1ng/ml. Conclusion: bFGF stimulated the proliferation of growth-plate chondrocytes obviously, but inhibited the differentiation of growth-plate chondrocytes at high concentrations.
%K Growth-plate Chondrocyte bFGF Proliferation Differentiation<br>生长板
%K 软骨细胞增殖
%K FGF
%K 分化
%K 碱性成纤维细胞生长因子
%K 抑制
%K 刺激
%K 养兔
%K 浓度
%K 产量
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=652EE71D11A63367&yid=2DD7160C83D0ACED&vid=C5154311167311FE&iid=0B39A22176CE99FB&sid=2A8D03AD8076A2E3&eid=B91E8C6D6FE990DB&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=3&reference_num=9