Expression of the Recombinant HSA-G-CSF in Pichia pastoris
重组HSA-hG-CSF融合蛋白在毕赤酵母中的表达

The complete gene of HSA-G-CSF was obtained by follow protocol: the HSA gene was obtained from the library of cDNA by PCR, and the gene of G-CSF was obtained from vector we constructed before. After ligation of the two genes, the complete gene was cloned to recombinant plasmid of pGENYD and then the linearized vector was transfected into Pichia pastoris. The target protein secreted by the recombinant Pichia pastoris was induced by methanol. The target protein showed single band in the SDS-PAGE. Western Blot showed the HSA-G-CSF combining activity both to HSA and G-CSF. The in intro activity showed that the activity of HSA-G-CSF is more than half of the G-CSF by the same molar ratio. And the animal study indicated the HSA-G-CSF have 15-20 multiple half-life in vivo than G-CSF.