%0 Journal Article
%T Expression of the Recombinant HSA-G-CSF in Pichia pastoris<br>重组HSA-hG-CSF融合蛋白在毕赤酵母中的表达
%A WANG Chang-mei
%A WANG Tong-ying
%A YANG Zhi-yu
%A SHAN Jian-feng
%A LIU Xiao-ni
%A MA Guo-chang
%A RONG Ya-wen
%A FANG Jing-jin
%A HUANG Yan-shan
%A ZHOU Jin-bao
%A <br>王昌梅
%A 王同映
%A 杨志愉
%A 单剑峰
%A 刘晓妮
%A 马国昌
%A 戎亚雯
%A 方井晋
%A 黄岩山
%A 周金宝
%J 中国生物工程杂志
%D 2006
%I 
%X The complete gene of HSA-G-CSF was obtained by follow protocol: the HSA gene was obtained from the library of cDNA by PCR, and the gene of G-CSF was obtained from vector we constructed before. After ligation of the two genes, the complete gene was cloned to recombinant plasmid of pGENYD and then the linearized vector was transfected into Pichia pastoris. The target protein secreted by the recombinant Pichia pastoris was induced by methanol. The target protein showed single band in the SDS-PAGE. Western Blot showed the HSA-G-CSF combining activity both to HSA and G-CSF. The in intro activity showed that the activity of HSA-G-CSF is more than half of the G-CSF by the same molar ratio. And the animal study indicated the HSA-G-CSF have 15-20 multiple half-life in vivo than G-CSF.
%K rHSA-G-CSF fusion protein Pichia pastoris Fusion expression<br>HSA-hG-CSF融合蛋白
%K 毕氏酵母
%K 融合表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=8C97ABF9012B122B&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=59906B3B2830C2C5&sid=339D79302DF62549&eid=7C3A4C1EE6A45749&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=3&reference_num=12