Construction of Plasmid pUC19K and Its Application in Brucella Mutant
pUC19K质粒的构建及其在布鲁氏菌突变株构建中的应用

Mutant construction is a prerequisite work in bacterial gene function studies.A new suicide plasmid was constructed to produce Brucella mutant.A kanamycin gene was inserted into the multi-cloning site of pUC19,and on either side of the resistance gene,several enzyme sites were inserted,resulting in the new plasmid pUC19K.Using this plasmid,a replacement mutant of Brucella omp25 gene was constructed.As the results showed,the mutant could be generated by only one round of selection with high efficiency.Therefore,the construction and application of pUC19K provides a new suicide plasmid for Brucella mutant construction and would accelerate gene function analysis of Brucella.