%0 Journal Article
%T Construction of Plasmid pUC19K and Its Application in Brucella Mutant<br>pUC19K质粒的构建及其在布鲁氏菌突变株构建中的应用
%A QIAO Feng
%A CHEN Ze-liang
%A WANG Yu-fei
%A ZHAO Jin
%A DU Xin-ying
%A YU Ya-qin
%A HUANG Liu-yu
%A <br>乔凤
%A 陈泽良
%A 王玉飞
%A 赵瑾
%A 杜昕颖
%A 于雅琴
%A 黄留玉
%J 中国生物工程杂志
%D 2007
%I 
%X Mutant construction is a prerequisite work in bacterial gene function studies.A new suicide plasmid was constructed to produce Brucella mutant.A kanamycin gene was inserted into the multi-cloning site of pUC19,and on either side of the resistance gene,several enzyme sites were inserted,resulting in the new plasmid pUC19K.Using this plasmid,a replacement mutant of Brucella omp25 gene was constructed.As the results showed,the mutant could be generated by only one round of selection with high efficiency.Therefore,the construction and application of pUC19K provides a new suicide plasmid for Brucella mutant construction and would accelerate gene function analysis of Brucella.
%K Brucella Suicide plasmid Mutant construction<br>布鲁氏菌
%K 自杀质粒
%K 突变株构建
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=46ADD96D0FACBC66DBB2DCDF812C8462&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=59906B3B2830C2C5&sid=CA4FD0336C81A37A&eid=94C357A881DFC066&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=9