Cloning and sequencing of gene encoding hyaluronidase from the venom of Apis mEllifera
意大利蜜蜂蜂毒透明质酸酶基因的克隆与序列分析

The venomous hyaluronidase (AmHya) gene of Italian honey bee ( Apis mellifera ) collected in China was amplified by RT PCR from total RNA of venom glands of the worker bee. Sequence analysis showed the gene was composed of 1 149 nucleotides . The alignment of the amino acid sequence of AmHya gene and 6 Hya genes from other organisms showed that AmHya is most closely related to the AcHya of Chinese honeybee ( A.cerana cerana ) with 91% amino acid level identity. A phylogenetic tree of 7 Hyaluronidases was drawn by using GENETYX program, and molecular structures and functions of these ensymes were compared and analysed.