%0 Journal Article
%T Cloning and sequencing of gene encoding hyaluronidase from the venom of Apis mEllifera<br>意大利蜜蜂蜂毒透明质酸酶基因的克隆与序列分析
%A SHEN Li rong
%A ZHANG Chuan xi
%A CHENG Jia an
%A <br>沈立荣
%A 张传溪
%A 程家安
%J 浙江大学学报(农业与生命科学版)
%D 2002
%I Zhejiang University Press
%X The venomous hyaluronidase (AmHya) gene of Italian honey bee ( Apis mellifera ) collected in China was amplified by RT PCR from total RNA of venom glands of the worker bee. Sequence analysis showed the gene was composed of 1 149 nucleotides . The alignment of the amino acid sequence of AmHya gene and 6 Hya genes from other organisms showed that AmHya is most closely related to the AcHya of Chinese honeybee ( A.cerana cerana ) with 91% amino acid level identity. A phylogenetic tree of 7 Hyaluronidases was drawn by using GENETYX program, and molecular structures and functions of these ensymes were compared and analysed.
%K Apis mellifera
%K bee venom
%K hyaluronidase
%K gene
%K sequence analysis<br>意大利蜜蜂
%K 蜂毒
%K 透明质酸酶基因
%K 克隆
%K 序列分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=521878027583A259&yid=C3ACC247184A22C1&vid=D3E34374A0D77D7F&iid=38B194292C032A66&sid=F50A8B5513721E1C&eid=8BB50A069C48D50B&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=1&reference_num=11