High Frequency Production of Doubled Haploid Rapeseed Plants by Direct Colchicine Treatment of Isolated Microspores
油菜小孢子秋水仙素直接处理增加纯合两倍体植株频率

Techniques for microspore culture of Brassica napus have been improved rapidly, and embryogenesis has been achieved in a wide range of genotypes. Plants regenerated from microspore-derived embryoids can be haploid, diploid or polyploid. From rapeseed microspore culture it is reported that 70%-90% of regenerated plants are haploid. The usual methods of chromosome doubling involve soaking roots (most common) or whole plants in a colchicine solution, or culturing plantlets in colchicine-containing medium in the greenhouse. Other alternatives are injecting colchicine into the secondary buds or applying colchicine-soaked cotton plugs to axillary buds.