%0 Journal Article
%T High Frequency Production of Doubled Haploid Rapeseed Plants by Direct Colchicine Treatment of Isolated Microspores<br>油菜小孢子秋水仙素直接处理增加纯合两倍体植株频率
%A ZHOU Wei-jun
%A HAGBERG Per
%A <br>周伟军
%A HAGBERG Per
%J 浙江大学学报(农业与生命科学版)
%D 2000
%I Zhejiang University Press
%X Techniques for microspore culture of Brassica napus have been improved rapidly, and embryogenesis has been achieved in a wide range of genotypes. Plants regenerated from microspore-derived embryoids can be haploid, diploid or polyploid. From rapeseed microspore culture it is reported that 70%-90% of regenerated plants are haploid. The usual methods of chromosome doubling involve soaking roots (most common) or whole plants in a colchicine solution, or culturing plantlets in colchicine-containing medium in the greenhouse. Other alternatives are injecting colchicine into the secondary buds or applying colchicine-soaked cotton plugs to axillary buds.
%K 油菜小孢子
%K 秋水仙素
%K 纯合两倍体
%K 植株频率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=A012AF728CB87322520CFB8247110901&yid=9806D0D4EAA9BED3&vid=96C778EE049EE47D&iid=0B39A22176CE99FB&sid=F122871CC7EC92DC&eid=6FBD78E3BAB60869&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=0