Optimization of human TAT-Fab antibody expression and its bioactivity assay
人源性TAT-Fab抗体的表达优化及生物活性测定

In order to establish high effective expression system for human TAT-Fab antibody with protective activity,the induction conditions for the expression of TAT-Fab antibody in E.coli were optimized,and the bioactivity of antibody was assayed in vitro and in vivo.ELISA showed that human TAT-Fab antibody expressed in E.coli possessed binding activity with tetanus toxin,and the expression level increased to nearly 5 times as compared with that before optimization.The expression products were ultra-filtered and analyzed by Western-blot,a specific band of TAT-Fab antibody,about 50 kDa,was detected.This antibody could neutralized tetanus toxin specifically and prolonged survival time of the mouse in vivo.The results indicate that human TAT-Fab antibody has the effect of neutralizing tetanus toxin in vitro and in vivo,which will provide experimental data for the production of human TAT antibody in the future.