%0 Journal Article
%T Optimization of human TAT-Fab antibody expression and its bioactivity assay<br>人源性TAT-Fab抗体的表达优化及生物活性测定
%A WANG Qun
%A LIU Jun-li
%A GAO Fei
%A ZHANG Pin
%A ZHANG Li-ning
%A <br>王群
%A 刘军莉
%A 高飞
%A 张蘋
%A 张利宁
%J 浙江大学学报(农业与生命科学版)
%D 2007
%I Zhejiang University Press
%X In order to establish high effective expression system for human TAT-Fab antibody with protective activity,the induction conditions for the expression of TAT-Fab antibody in E.coli were optimized,and the bioactivity of antibody was assayed in vitro and in vivo.ELISA showed that human TAT-Fab antibody expressed in E.coli possessed binding activity with tetanus toxin,and the expression level increased to nearly 5 times as compared with that before optimization.The expression products were ultra-filtered and analyzed by Western-blot,a specific band of TAT-Fab antibody,about 50 kDa,was detected.This antibody could neutralized tetanus toxin specifically and prolonged survival time of the mouse in vivo.The results indicate that human TAT-Fab antibody has the effect of neutralizing tetanus toxin in vitro and in vivo,which will provide experimental data for the production of human TAT antibody in the future.
%K Fab<br>破伤风抗毒素
%K 噬菌体展示
%K 抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=929E400BAAD1E26F852D1626AFA3E834&yid=A732AF04DDA03BB3&vid=27746BCEEE58E9DC&iid=B31275AF3241DB2D&sid=7004BE6E41AAF52C&eid=04FC77FB58A9B53A&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=12