Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003
以潮霉素抗性为选择标记的毛壳霉原生质体转化

Genetic transformation has been achieved by incubatingprotoplasts from Chaetomium globosum with plasmid PCB1003. The efficiency was 1-2 transformants/μg vector DNA. Protoplasts were produced by treating 14-hour old mycelium of Chaetomium globosum with 10 mg/mL lysozyme. Chaetomium globosum growth was inhibited completely when the medium contained 150 μg/mL hygromycin B. Integration of hph gene was verified by PCR and PCR product hybridization analysis. There was no evidence of mitotic of hygromycin B resistance in the transformants.