%0 Journal Article
%T Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003<br>以潮霉素抗性为选择标记的毛壳霉原生质体转化
%A CHEN Zhen ming
%A GUO Ze jian
%A LIN Fu cheng
%A LI De bao
%A <br>陈振明
%A 郭泽建
%A 林福呈
%A 李德葆
%J 浙江大学学报(农业与生命科学版)
%D 2001
%I Zhejiang University Press
%X Genetic transformation has been achieved by incubatingprotoplasts from Chaetomium globosum with plasmid PCB1003. The efficiency was 1-2 transformants/μg vector DNA. Protoplasts were produced by treating 14-hour old mycelium of Chaetomium globosum with 10 mg/mL lysozyme. Chaetomium globosum growth was inhibited completely when the medium contained 150 μg/mL hygromycin B. Integration of hph gene was verified by PCR and PCR product hybridization analysis. There was no evidence of mitotic of hygromycin B resistance in the transformants.
%K Chaetomium globosum
%K transformation
%K hygromycin B resistance<br>毛壳霉
%K 潮霉素抗体
%K 原生质体转化
%K 选择标记
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=E4BFA1A06904A047&yid=14E7EF987E4155E6&vid=DB817633AA4F79B9&iid=CA4FD0336C81A37A&sid=2A8D03AD8076A2E3&eid=BC12EA701C895178&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=9&reference_num=13