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Effect of STZ-induced diabetes on bone mineral density and testosterone
STZ诱导的糖尿病对骨密度和血清睾酮的影响(英文)

Keywords: osteoporosis,diabetes mellitus,bone mineral density,testosterone
骨质疏松
,糖尿病,骨密度,睾酮

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Abstract:

Toinvestigate the effect of diabetes mellitus on bone metabolism,bone mineral density(BMD) and serumtestosterone were studied in STZ-induced diabetic male rats.Male Wistar rats were randomly divided into control group(n=6),diabetic group(n=5) and insulin-treated diabetic group(n=5).Diabetes was intravenously induced by injection of streptozotocin(STZ,50mg/kg body weight),confirmed by 12h fasting blood glucose concentration(12mmol/L).The treatment group was subcutaneously injected with protamine zinc insulin(1.8 ~2.2U/animal)at the same time every day.The experiment lasted 32d.Femoral BMD was determined by dual-energy X-ray absorptiometry(DEXA).Serumtestosterone was detected by Enzyme-linked Immunosorbent Assay(ELISA)kit.Total alkaline phosphatase(ALP),calcium (Ca) and phosphorus(P)in serum were measured by bio-machine.Femoral BMDin diabetic group was significantly lower than that in control(P<0.01) but reserved by insulin treatment.Testosterone in serum was also reducedin diabetic rats(P<0.01) and normalized byinsulintreatment.The serum ALP concentration was markedly higher in diabetics(P<0.01) compared with the controls and prevented by insulin treatment.However,no significant differences were observed in serum Ca and P among threegroups.Our results suggest that BMDis seriously affected by diabetes.Insulin deficiency and testosterone decrease are important causes of diabetic osteoporosis.And insulin treatment prevented bone loss and testosterone depression.

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