Influence of Taxol Production by Product Extraction Strategies in Cell Cultures of Taxus chinensis
红豆杉细胞培养产物提取策略及其对紫杉醇的影响

The effects of product release and in situ extraction on cell growth and taxol release and accumulation in cell suspension cultures of Taxus chinensis were investigated. Addition of 2 mg/L ammonium cerous sulphate on day 12 gave the greatest taxol production at 8 3 mg/L, among which 2 4 mg/L was released out of the cell, which was 4 and 12 times that of the control culture. Extracellular taxol production reached 9 mg/L when 2 mg/L ammonium cerous sulphate and 5% oleic acid (v/v) were added together, which was 45 times that of the control culture. The highest taxol accumulation of 24.5 mg/L with the release ratio of 60% was obtained when 2 mg/L ammonium cerous sulphate, 5% oleic acid (v/v) and feeding solution with 20 g/L sucrose were added.