%0 Journal Article
%T Influence of Taxol Production by Product Extraction Strategies in Cell Cultures of Taxus chinensis<br>红豆杉细胞培养产物提取策略及其对紫杉醇的影响
%A LUO Jie
%A LIU Ling
%A HU Dao Wei
%A <br>罗杰
%A 刘凌
%A 胡道伟
%J 植物科学学报
%D 2003
%I 
%X The effects of product release and in situ extraction on cell growth and taxol release and accumulation in cell suspension cultures of Taxus chinensis were investigated. Addition of 2 mg/L ammonium cerous sulphate on day 12 gave the greatest taxol production at 8 3 mg/L, among which 2 4 mg/L was released out of the cell, which was 4 and 12 times that of the control culture. Extracellular taxol production reached 9 mg/L when 2 mg/L ammonium cerous sulphate and 5% oleic acid (v/v) were added together, which was 45 times that of the control culture. The highest taxol accumulation of 24.5 mg/L with the release ratio of 60% was obtained when 2 mg/L ammonium cerous sulphate, 5% oleic acid (v/v) and feeding solution with 20 g/L sucrose were added.
%K Taxus
%K Taxol
%K Ammonium cerous sulphate
%K in situ extraction<br>红豆杉
%K 紫杉醇
%K 硫酸铈铵
%K 原位提取
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=E5A710DB8AD0482D&yid=D43C4A19B2EE3C0A&vid=659D3B06EBF534A7&iid=0B39A22176CE99FB&sid=31611641D4BB139F&eid=954CE65414DD94CA&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=0&reference_num=10