Effects of Medium Concentration on Callus Growth and Secondary Metabolites of Eucommia ulmoides
培养基成分对杜仲愈伤组织生长及次生代谢产物含量的影响

The effects of medium concentration on callus growth and the accumulation of chlorogenic acid and flavonoids of Eucommia ulmoides were investigated. The treatments were designed in B_5 medium supplemented with 0.5 mg/L NAA + 0.5 mg/L BA.The results showed that the callus growth was increased when KNO_3 content was increased,whereas chlorogenic acid and flavonoids contents were highest when KNO_3 concentration was 2/3 of original amount in B_5 medium.The 4/3 content of (NH_4)_2SO_4 in B_5 medium were best for the callus growth and flavonoids content and yield, whereas chlorogenic acid content was highest when (NH_4)_2SO_4 content was 1/3 of original amount in B_5 medium.The callus growth was highest when MgSO_4 concentration was 2/3,whereas chlorogenic acid and flavonoids contents were highest when MgSO_4 content was 1/3 in B_5 medium. The original content of NaH_2PO_4,CaCl_2 and MnSO_4 in B_5 medium were best for the callus growth and the accumulation of chlorogenic acid and flavonoids. The contents of ZnSO_4 and FeSO_4 in B_5 medium was best for the callus growth but chlorogenic acid and flavonoids contents was highest when ZnSO_4 and FeSO_4 concentration was 1/3 in B_5 medium.