%0 Journal Article
%T Effects of Medium Concentration on Callus Growth and Secondary Metabolites of Eucommia ulmoides
培养基成分对杜仲愈伤组织生长及次生代谢产物含量的影响
%A LI Yan
%A ZHANG Zhao-Hong
%A MA Xi-Han
%A
李琰
%A 张朝红
%A 马希汉
%J 植物科学学报
%D 2004
%I
%X The effects of medium concentration on callus growth and the accumulation of chlorogenic acid and flavonoids of Eucommia ulmoides were investigated. The treatments were designed in B_5 medium supplemented with 0.5 mg/L NAA + 0.5 mg/L BA.The results showed that the callus growth was increased when KNO_3 content was increased,whereas chlorogenic acid and flavonoids contents were highest when KNO_3 concentration was 2/3 of original amount in B_5 medium.The 4/3 content of (NH_4)_2SO_4 in B_5 medium were best for the callus growth and flavonoids content and yield, whereas chlorogenic acid content was highest when (NH_4)_2SO_4 content was 1/3 of original amount in B_5 medium.The callus growth was highest when MgSO_4 concentration was 2/3,whereas chlorogenic acid and flavonoids contents were highest when MgSO_4 content was 1/3 in B_5 medium. The original content of NaH_2PO_4,CaCl_2 and MnSO_4 in B_5 medium were best for the callus growth and the accumulation of chlorogenic acid and flavonoids. The contents of ZnSO_4 and FeSO_4 in B_5 medium was best for the callus growth but chlorogenic acid and flavonoids contents was highest when ZnSO_4 and FeSO_4 concentration was 1/3 in B_5 medium.
%K Eucommia ulmoides
%K Tissue culture
%K Salt
%K Secondary metabolites
杜仲
%K 组织培养
%K 无机盐
%K 次生代谢产物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=927A666FF0367881&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=E158A972A605785F&sid=3356A7630A93A219&eid=8C27CCA578E52082&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=8&reference_num=14