Cloning and Bioinformatics Analysis of TIR1 cDNA from Super Hybrid Rice Parental Line 1S
超级杂交水稻 TIR1 类似基因 cDNA 的克隆与生物信息学分析

The auxin receptor TIR1, which can directly bind to auxin through the formation of the SCFTIR1 complex , is the key protein in the Aux /IAA degradation pathway of the 26S proteasome. In this research, we designed a specific primer according to the results of a BLAST search of bioinformatics data; the super hybrid rice parental line 1S was used as the plant material. After RT-PCR and T-A cloning, a 2 219 bp cDNA sequence was obtained through sequencing. Its open reading frame (ORF) was 1 764 bp long and coded for a polypeptide of 587 amino acids. Bioinformatics analysis revealed that the polypeptide shares 77% similarity with AtTIR1. Both polypeptides contain an F-box motif and a leucine-rich repeat domain but no transmembrane helices or signal peptide. The cDNA sequence was designated as OsTIR1 and submitted to GenBank.