%0 Journal Article
%T Cloning and Bioinformatics Analysis of TIR1 cDNA from Super Hybrid Rice Parental Line 1S<br>超级杂交水稻 TIR1 类似基因 cDNA 的克隆与生物信息学分析
%A Zhigang Huang
%A Huang Hu
%A Xuanzhi Zhao
%A Ruozhong Wang
%A Langtao Xiao
%A <br>黄志刚
%A 胡皝
%A 赵玄之
%A 王若仲
%A 萧浪涛
%J 植物学报
%D 2008
%I 
%X The auxin receptor TIR1, which can directly bind to auxin through the formation of the SCFTIR1 complex , is the key protein in the Aux /IAA degradation pathway of the 26S proteasome. In this research, we designed a specific primer according to the results of a BLAST search of bioinformatics data; the super hybrid rice parental line 1S was used as the plant material. After RT-PCR and T-A cloning, a 2 219 bp cDNA sequence was obtained through sequencing. Its open reading frame (ORF) was 1 764 bp long and coded for a polypeptide of 587 amino acids. Bioinformatics analysis revealed that the polypeptide shares 77% similarity with AtTIR1. Both polypeptides contain an F-box motif and a leucine-rich repeat domain but no transmembrane helices or signal peptide. The cDNA sequence was designated as OsTIR1 and submitted to GenBank.
%K <br>生长素受体
%K 生物信息学
%K 超级杂交水稻
%K TIR1
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=44479BC4B4BB1945F1A3A47851EBF1C0&yid=67289AFF6305E306&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=08B2E838F29A693A&eid=27A3D4EE1F52A91C&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=0