Quantitative Analysis of CHS Gene Expression in Arabidopsis thaliana by Real-time RT-PCR
拟南芥CHS 基因表达的实时荧光定量PCR 检测

Fluorescent-based real-time PCR is widely used for quantification of nucleic acids. It is becoming one of the most important techniques for molecular biology and biotechnology.Real-time PCR assay is easy to perform, capable of high throughput, and can combine high sensitivity with reliable specificity. The technique is evolving rapidly with the knowledge of new enzymes, chemistry and instrumentation. The principle of real-time PCR and its application in quantifying plant nucleic acids is reviewed.