%0 Journal Article
%T Quantitative Analysis of CHS Gene Expression in Arabidopsis thaliana by Real-time RT-PCR<br>拟南芥CHS 基因表达的实时荧光定量PCR 检测
%A WANG Yan JIANG Lei LI Shao-Shan
%A <br>王艳
%A 蒋磊
%A 李韶山
%J 植物学报
%D 2005
%I 
%X Fluorescent-based real-time PCR is widely used for quantification of nucleic acids. It is becoming one of the most important techniques for molecular biology and biotechnology.Real-time PCR assay is easy to perform, capable of high throughput, and can combine high sensitivity with reliable specificity. The technique is evolving rapidly with the knowledge of new enzymes, chemistry and instrumentation. The principle of real-time PCR and its application in quantifying plant nucleic acids is reviewed.
%K <br>拟南芥
%K 查耳酮合成酶基因
%K 基因表达定量分析
%K 实时荧光定量PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=27A88E70D44B37C91159C107586C9CF6&yid=2DD7160C83D0ACED&vid=BC12EA701C895178&iid=94C357A881DFC066&sid=65FC738C50B41E43&eid=4720E9D07E8A2290&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=0