Transient expression of hepatitis B virus surface antigen (HBsAg) and human erythropoietin (hEPO) genes in milk after direct introduction into ewe

The ability to deliver nonviral DNA dtrectly into animals would provide major practical and economic benefits. To pursue this potential, as a preliminary approach, the expression in mammary epithelial cells with nonviral DNA ending HBsAg and hEPO was investigated byin vivo delivery directly into mammary gland Either the bovine asl-casein-HBsAg or the WAE-hEPO gene construct was introduced into mammary glands of dm by mammary artery injection. A single injection of 1011 copies of DNA construct was implemented for a doe. Transient expression of the constructs in milk collected from milk letdown by induction up to 19 d after injection was measured with an hEPO ELISA kit from Boehringer Mannheim (Cat. No. 1 693 417) for rhEPO, and a HBsAg-ELISA kit (Abbott Lab. USA) for rHBsAg. The treated animals showed mean concentration of recombinant molecules at 16 μg/L for rHEisAg, and 2 mg/L for rhEPO in milk. Project supported by the National Gongguan Program.