%0 Journal Article
%T Transient expression of hepatitis B virus surface antigen (HBsAg) and human erythropoietin (hEPO) genes in milk after direct introduction into ewe<br>
%A LAO Weide
%A <br>
%J 中国科学C辑(英文版)
%D 1998
%I Springer
%X The ability to deliver nonviral DNA dtrectly into animals would provide major practical and economic benefits. To pursue this potential, as a preliminary approach, the expression in mammary epithelial cells with nonviral DNA ending HBsAg and hEPO was investigated byin vivo delivery directly into mammary gland Either the bovine asl-casein-HBsAg or the WAE-hEPO gene construct was introduced into mammary glands of dm by mammary artery injection. A single injection of 1011 copies of DNA construct was implemented for a doe. Transient expression of the constructs in milk collected from milk letdown by induction up to 19 d after injection was measured with an hEPO ELISA kit from Boehringer Mannheim (Cat. No. 1 693 417) for rhEPO, and a HBsAg-ELISA kit (Abbott Lab. USA) for rHBsAg. The treated animals showed mean concentration of recombinant molecules at 16 μg/L for rHEisAg, and 2 mg/L for rhEPO in milk. Project supported by the National Gongguan Program.
%K mammary artery injection
%K transient expression
%K in vivo
%K nonviral DNA introduction
%K rHBsAg
%K rhEPO<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=6C74CE0A7BCCDAD8E8775446EB2A6853&yid=8CAA3A429E3EA654&vid=2001E0D53B7B80EC&iid=94C357A881DFC066&sid=BC88D6B0750E09D1&eid=7D1E6EEC2019967D&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=1&reference_num=13