Establishment of Embryogenic Cell Suspension Culture and Plant Regeneration of Rare Endangered Plant Emmenopterys henryi
珍稀濒危植物香果树胚性细胞悬浮系的建立和植株再生

Embry ogenic cell suspension cultures were established from calli of immature seeds of Emmenopterys henryi. Callus could be obtained from immature seeds on MS solid medium augmented with 2.0 mg·L-1 6-benz ylaminopur ine (6-BA) and 3% sucrose. Embryogenic calli were induced on MS basal medium supplemented with 0.1 mg·L-1 N-acetylaspartate (NAA) and 0.5 mg·L-1 6-BA. Numerous somatic embryoids appeared on MS liquid basal nutrient medium with 0.5 mg·L-1 NAA and 0.5 mg·L-1 6-BA. The optimal value of the initial cell density was 2.0% (fresh weight). Several parameters that might be as sociated with growth were determined over a 14-day period. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse, with a high survival rate of 95%.