%0 Journal Article
%T Establishment of Embryogenic Cell Suspension Culture and Plant Regeneration of Rare Endangered Plant Emmenopterys henryi<br>珍稀濒危植物香果树胚性细胞悬浮系的建立和植株再生
%A Dan Xiong
%A Faju Chen
%A Hongwei Liang
%A Yubing Wang
%A <br>熊丹
%A 陈发菊
%A 梁宏伟
%A 王玉兵
%J 植物学报
%D 2008
%I 
%X Embry ogenic cell suspension cultures were established from calli of immature seeds of Emmenopterys henryi. Callus could be obtained from immature seeds on MS solid medium augmented with 2.0 mg·L-1 6-benz ylaminopur ine (6-BA) and 3% sucrose. Embryogenic calli were induced on MS basal medium supplemented with 0.1 mg·L-1 N-acetylaspartate (NAA) and 0.5 mg·L-1 6-BA. Numerous somatic embryoids appeared on MS liquid basal nutrient medium with 0.5 mg·L-1 NAA and 0.5 mg·L-1 6-BA. The optimal value of the initial cell density was 2.0% (fresh weight). Several parameters that might be as sociated with growth were determined over a 14-day period. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse, with a high survival rate of 95%.
%K <br>胚性悬浮细胞
%K 香果树
%K 植株再生
%K 体细胞胚胎发生
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=D03FB8974A42DCBE5FD0FEE4BDFB330F&yid=67289AFF6305E306&vid=C5154311167311FE&iid=38B194292C032A66&sid=5FF9F4F7CB1800C7&eid=5957D6E0A50D26B5&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=8