Introduction of bioluminescence genes into silicate-dissolving bacteria strain NBT
发光酶基因lux AB标记硅酸盐细菌NBT菌株的研究

In this study, silicate-dissolving bacteria NBT strain was grown with 0.25% maltose as carbon source, and the rifampicin-resistance was generated by ultraviolet mutagenesis and streak naturalized to 200 microg x ml(-1). Through triparental cross, the luxAB genes were introduced into NBT-R200 at the help of pRK2013. The luminescence activity of the hybrid strain was tested, which indicated that all colonies had a high luminescence activity and kanamycin-, tetracyctine-and rifampicin-resistance. The NBT-R200 cells prepared from initial logarithmic growth phage were more likely to be sensitive to foreign DNA, and the maximum translocation frequency was up to 6.70 x 10(-5). In addition, the optimal mating ratio was 1:1:1. The potassium release ability from feldspar and the luminescence of luxAB genes marked silicate-dissolving bacteria RL85 strain were stable, and hence, the RL85 was available to rhizosphere microecology researches.