%0 Journal Article
%T Introduction of bioluminescence genes into silicate-dissolving bacteria strain NBT<br>发光酶基因lux AB标记硅酸盐细菌NBT菌株的研究
%A HE Linyan
%A HUANG Weiyi
%A <br>何琳燕
%A 黄为一
%J 应用生态学报
%D 2004
%I 
%X In this study, silicate-dissolving bacteria NBT strain was grown with 0.25% maltose as carbon source, and the rifampicin-resistance was generated by ultraviolet mutagenesis and streak naturalized to 200 microg x ml(-1). Through triparental cross, the luxAB genes were introduced into NBT-R200 at the help of pRK2013. The luminescence activity of the hybrid strain was tested, which indicated that all colonies had a high luminescence activity and kanamycin-, tetracyctine-and rifampicin-resistance. The NBT-R200 cells prepared from initial logarithmic growth phage were more likely to be sensitive to foreign DNA, and the maximum translocation frequency was up to 6.70 x 10(-5). In addition, the optimal mating ratio was 1:1:1. The potassium release ability from feldspar and the luminescence of luxAB genes marked silicate-dissolving bacteria RL85 strain were stable, and hence, the RL85 was available to rhizosphere microecology researches.
%K Silicate-dissolving bacteria
%K lux marker genes
%K Triparental cross<br>硅酸盐细菌
%K 发光酶基因
%K 标记
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=42FEDDFE54F8BCD9D5FD1926ABDD7722&aid=1BEEC19A93C2A27B&yid=D0E58B75BFD8E51C&vid=23CCDDCD68FFCC2F&iid=DF92D298D3FF1E6E&sid=A3FC76ED9EF62E85&eid=3559FBE006544B4B&journal_id=1001-9332&journal_name=应用生态学报&referenced_num=3&reference_num=25