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OALib Journal期刊
ISSN: 2333-9721
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遗传  2002 

Rapid Extraction of MtDNA from Cryptococcus neoformans and Examined by Electronic Microscopy
新生隐球菌线粒体的快速抽提和电镜观察 Rapid Extraction of MtDNA from Cryptococcus neoformans and Examined by Electronic Microscopy

Keywords: Cryptococcus neoformans,mitochondria,DNA,ultrastructure
新生隐球菌
,线粒体DNA,超微结构,快速抽提

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Abstract:

Cryptococcus neoformans may be grown to the exponential phase,are broken by a combination of NovoZym234 and mechanical means,and mitochondrial DNA was extracted from DNaseI-treated mitrochondrial preparetion by differential centrifugation.Three pellets,including yeast cell,protoplasts,mitochondrial,were examined by transmission electronic microscopy.The resulting mtDNA is sufficicently pure for restriction endonucleases analysis and PCR in further studying.A rapid and effective method for the preparation of the mtDNA of C.neoformans was established.

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